Difference between revisions of "Part:BBa K121012"
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<partinfo>BBa_K121012 short</partinfo>
 
<partinfo>BBa_K121012 short</partinfo>
  
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pressure inducible Plac GFP reporter
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pressure and IPTG inducible Plac GFP reporter
  
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
'''Identify Hill coefficient by IPTG induction'''
 +
----
 +
 +
Plac is known to be induced by IPTG. Using iGEM2007 Tokyo_Tech assay protocol, we identified hill coefficient of Plac_GFP in BBa_I739003 in
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 +
''E. Coli'' strain JM109.
 +
 
[[Image:Transfer Function K121012.jpg]]
 
[[Image:Transfer Function K121012.jpg]]
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 +
The hill coefficient is 2.15.
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 +
'''Pressure induction'''
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----
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 +
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
  

Revision as of 14:37, 22 October 2008

Plac GFP on pSB6

pressure and IPTG inducible Plac GFP reporter

Usage and Biology

Identify Hill coefficient by IPTG induction

Plac is known to be induced by IPTG. Using iGEM2007 Tokyo_Tech assay protocol, we identified hill coefficient of Plac_GFP in BBa_I739003 in

E. Coli strain JM109.

Transfer Function K121012.jpg

The hill coefficient is 2.15.

Pressure induction


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 267
    Illegal BamHI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 954