Difference between revisions of "Part:BBa K3113051"
Theresakeil (Talk | contribs) |
Theresakeil (Talk | contribs) |
||
| Line 8: | Line 8: | ||
<h3>6 histidines were added after position Ser161 based on a structural model for CD63 generated with swissmodel.expasy.org</h3> | <h3>6 histidines were added after position Ser161 based on a structural model for CD63 generated with swissmodel.expasy.org</h3> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col-12 col-md- | + | <div class="col-12 col-md-3"> |
<figure class="figure"> | <figure class="figure"> | ||
<img src="https://2019.igem.org/wiki/images/c/cb/T--Munich--Improved_CD63.png" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure."> | <img src="https://2019.igem.org/wiki/images/c/cb/T--Munich--Improved_CD63.png" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure."> | ||
Revision as of 11:42, 20 October 2019
CD63_Ser161_6xHis
To facilitate the purification of intact exosomes, we engineered the exosomal marker CD63 by integrating a polyhistidine repeat into the large extracellular loop of the tetraspanin. Based on SWISS-MODEL, a protein structure homology-modelling server, we were able to model CD63 and to search for sites within CD63 that are accessible.
6 histidines were added after position Ser161 based on a structural model for CD63 generated with swissmodel.expasy.org
<figure class="figure">
<img src="
" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
</figure>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]