Difference between revisions of "Part:BBa K3113051"

Line 8: Line 8:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 
+
<h3>6 histidines were added after position Ser161 based on a structural model for CD63 generated with swissmodel.expasy.org</h3>
=6 histidines were added after position Ser161 based on a structural model for CD63 generated with swissmodel.expasy.org=
+
<div class="row">
<div class="white-box">
+
<div class="col-12 col-md-12">
[[https://2019.igem.org/wiki/images/c/cb/T--Munich--Improved_CD63.png|thumb|600px|right]]
+
<figure class="figure">
 +
<img src="https://2019.igem.org/wiki/images/c/cb/T--Munich--Improved_CD63.png" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 +
</figure>
 
</div>
 
</div>
 +
</div>
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 11:41, 20 October 2019


CD63_Ser161_6xHis

To facilitate the purification of intact exosomes, we engineered the exosomal marker CD63 by integrating a polyhistidine repeat into the large extracellular loop of the tetraspanin. Based on SWISS-MODEL, a protein structure homology-modelling server, we were able to model CD63 and to search for sites within CD63 that are accessible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]