Difference between revisions of "Part:BBa K3113051"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | =6 histidines were added after position Ser161 based on a structural model for CD63 generated with swissmodel.expasy.org= | |
| − | + | <div class="white-box"> | |
| − | https://2019.igem.org/wiki/images/c/cb/T--Munich--Improved_CD63.png | + | [[https://2019.igem.org/wiki/images/c/cb/T--Munich--Improved_CD63.png|thumb|600px|right]] |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 11:36, 20 October 2019
CD63_Ser161_6xHis
To facilitate the purification of intact exosomes, we engineered the exosomal marker CD63 by integrating a polyhistidine repeat into the large extracellular loop of the tetraspanin. Based on SWISS-MODEL, a protein structure homology-modelling server, we were able to model CD63 and to search for sites within CD63 that are accessible.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]