Difference between revisions of "Part:BBa K2983050"
Line 6: Line 6:
 
It is an improvement of [[Part:BBa_K2117000|BBa_K2117000]] as it has a mutated BsaI site (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
 
It is an improvement of [[Part:BBa_K2117000|BBa_K2117000]] as it has a mutated BsaI site (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
TEF is an isolated promoteur isolated with highly expressed genes in ''yarrowia lipolytica'' and present in only on copy in his genome. TEF encode for translation elongation factor-1 α.
 +
 +
<!-- Add more about the biology of this part here
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 11:30, 20 October 2019


pTef1c: BBa_K2117000 without the BsaI site

This part is a version of the Yarrowia lipolytica pTef1 promoter. It is an improvement of BBa_K2117000 as it has a mutated BsaI site (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.

Usage and Biology

TEF is an isolated promoteur isolated with highly expressed genes in yarrowia lipolytica and present in only on copy in his genome. TEF encode for translation elongation factor-1 α.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]