Difference between revisions of "Part:BBa K3113051"
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<partinfo>BBa_K3113051 short</partinfo>
 
<partinfo>BBa_K3113051 short</partinfo>
  
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CD63 is a tetraspanin found in exosomes. This version has a His-Tag cloned into the large extracellular loop at the position 161 for purification of exosomes.
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To facilitate the purification of intact exosomes, we engineered the exosomal marker CD63 by integrating a polyhistidine repeat into the large extracellular loop of the tetraspanin.
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Based on SWISS-MODEL, a protein structure homology-modelling server, we were able to model CD63 and to search for sites within CD63 that are accessible.
  
 
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===Usage and Biology===
 
===Usage and Biology===
  
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https://2019.igem.org/wiki/images/c/cb/T--Munich--Improved_CD63.png
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:29, 20 October 2019


CD63_Ser161_6xHis

To facilitate the purification of intact exosomes, we engineered the exosomal marker CD63 by integrating a polyhistidine repeat into the large extracellular loop of the tetraspanin. Based on SWISS-MODEL, a protein structure homology-modelling server, we were able to model CD63 and to search for sites within CD63 that are accessible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]