Difference between revisions of "Part:BBa K2963023"

Latest revision as of 11:16, 20 October 2019

BsracE200 - Bicistronic containing RBS200 to stabilize gene expression.

Usage and Biology

The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the racE gene, our team worked with the master in our lab to further stabilize the expression of the racE gene by using the bicistronic system.

Reference

Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 996
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2963023 short</partinfo>
-According to that pre-cistron can affect the expression of next cistron, and we select a short 5' sequence of high-protein-abundance gene from Corynebacterium glutamicum as the pre-cistron trying to improve the stabilization of the expression of racE(coding racemase enzyme).
+===Usage and Biology===
+The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the <i>racE</i> gene, our team worked with the master in our lab to further stabilize the expression of the <i>racE</i> gene by using the bicistronic system.
+===Reference===
+Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, <i>Corynebacterium glutamicum</i>." Engineering in Life Sciences (2017).
 <!-- Add more about the biology of this part here