Difference between revisions of "Part:BBa K2963014"

 
 
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<partinfo>BBa_K2963014 short</partinfo>
 
<partinfo>BBa_K2963014 short</partinfo>
  
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According to that pre-cistron can affect the expression of next cistron, and we select a short 5' sequence of high-protein-abundance gene from Corynebacterium glutamicum as the pre-cistron trying to improve the stabilization of the expression of racE(coding racemase enzyme).
 
  
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===Usage and Biology===
 
===Usage and Biology===
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The bicistronic has a translational coupling effect. Use bicistronic can stabilize the expression of the target gene. In order to more precisely regulate the activity of the <i>racE</i> gene, we wanted to stabilize the expression of <i> racE</i> gene by using bicistronic system.
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===Reference===
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Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, <i>Corynebacterium glutamicum</i>." Engineering in Life Sciences (2017).
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Latest revision as of 11:12, 20 October 2019


BsracE18 - Bicistronic containing RBS18 to stabilize gene expression.


Usage and Biology

The bicistronic has a translational coupling effect. Use bicistronic can stabilize the expression of the target gene. In order to more precisely regulate the activity of the racE gene, we wanted to stabilize the expression of racE gene by using bicistronic system.

Reference

Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 132
    Illegal NheI site found at 999
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 132
  • 1000
    COMPATIBLE WITH RFC[1000]