Difference between revisions of "Part:BBa K2980013"
Billsfriend (Talk | contribs) |
Billsfriend (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2980013 short</partinfo> | <partinfo>BBa_K2980013 short</partinfo> | ||
| − | This part can induce asymmetric cell division in E.coli. FUSLCD in this part would aggregate at one end of cells when expressing at proper level. At the same time, lacI could attach target plasmids with lacO on it, recruiting it to the end of cell with FUSLCD. As the plasmids attracted to an end, the two filial cells would differ in the copy number of target plasmid. | + | This part can induce asymmetric cell division in ''E.coli''. FUSLCD in this part would aggregate at one end of cells when expressing at proper level. At the same time, lacI could attach target plasmids with lacO on it, recruiting it to the end of cell with FUSLCD. As the plasmids attracted to an end, the two filial cells would differ in the copy number of target plasmid. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 11:04, 20 October 2019
lacI-GFP-FUS
This part can induce asymmetric cell division in E.coli. FUSLCD in this part would aggregate at one end of cells when expressing at proper level. At the same time, lacI could attach target plasmids with lacO on it, recruiting it to the end of cell with FUSLCD. As the plasmids attracted to an end, the two filial cells would differ in the copy number of target plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1377
Illegal NheI site found at 1400 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]