Difference between revisions of "Part:BBa K3202030"

 
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This composite part is designed as a genetic circuit that is used as a negative control group of out later experiment.
 
This composite part is designed as a genetic circuit that is used as a negative control group of out later experiment.
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In our study, we aimed to produce a system in which the coding sequence in the middle of the bistable system can be completely concealed. That is, there is no leakage during the normal phase of the functioning genetic circuit. Therefore, this composite part that encodes the AraC-pC-pBAD-sfGFP is used as a reference of what the bacterial colony should be like when there exists leakage, which means our design of the "lock" system failed.
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In our study, we made a comparison between this composite part to the efficiency of BBa_K3202029, and a comparison between BBa_K3202029 and BBa_K3202031
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:44, 20 October 2019


AraC-Pc-pBAD-sfGFP

This composite part is designed as a genetic circuit that is used as a negative control group of out later experiment.

In our study, we made a comparison between this composite part to the efficiency of BBa_K3202029, and a comparison between BBa_K3202029 and BBa_K3202031

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1252