Difference between revisions of "Part:BBa K3196025"
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<partinfo>BBa_K3196025 short</partinfo> | <partinfo>BBa_K3196025 short</partinfo> | ||
− | The SUC2 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. VP can catalyze | + | The SUC2 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. VP can catalyze lignin. |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
− | This is a four section for degrade and transfer lignin part. | + | This is a four section for degrade and transfer lignin part.The expression of SUC2 gene is a kind of Sucrose transferase [1], and its signal peptide can help the protein out cells. We use this signal peptide raise the efficiency of VP’s transformation.[[ File:T--HUST--China--2019-SUC2VP.jpg|400px|thumb|center|Figure1. T--HUST--China--2019-SUC2VP]] |
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− | <h1>''' | + | <h1>'''Reference'''</h1><!-- --> |
− | + | [1]Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzyme biochemical properties. Nooshin Mohandesi, Seyed Omid Ranaei Siadat, Kamahldin Haghbeen, Ardeshir Hesampour Received: 8 February 2016 / Accepted: 25 May 2016 / Published online: 8 June 2016 The Author(s) 2016. | |
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<partinfo>BBa_K3196025 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3196025 SequenceAndFeatures</partinfo> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Latest revision as of 10:40, 20 October 2019
AOX1-Kozak-SUC2-VP-His tag-AOX1 Terminator
The SUC2 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. VP can catalyze lignin.
Characterization
This is a four section for degrade and transfer lignin part.The expression of SUC2 gene is a kind of Sucrose transferase [1], and its signal peptide can help the protein out cells. We use this signal peptide raise the efficiency of VP’s transformation.
Reference
[1]Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzyme biochemical properties. Nooshin Mohandesi, Seyed Omid Ranaei Siadat, Kamahldin Haghbeen, Ardeshir Hesampour Received: 8 February 2016 / Accepted: 25 May 2016 / Published online: 8 June 2016 The Author(s) 2016.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 937
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]