Revision as of 13:10, 16 October 2019 (view source) Registry (Talk | contribs)		Latest revision as of 10:40, 20 October 2019 (view source) Satirev (Talk | contribs)
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	<partinfo>BBa_K3196025 short</partinfo>		<partinfo>BBa_K3196025 short</partinfo>
−	protein	+	The SUC2 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. VP can catalyze lignin.
−	<!-- Add more about the biology of this part here	+	<h1>'''Characterization'''</h1>
−	===Usage and Biology===	+	This is a four section for degrade and transfer lignin part.The expression of SUC2 gene is a kind of Sucrose transferase [1], and its signal peptide can help the protein out cells. We use this signal peptide raise the efficiency of VP’s transformation.[[ File:T--HUST--China--2019-SUC2VP.jpg‎|400px|thumb|center|Figure1. T--HUST--China--2019-SUC2VP]]
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−	<span class='h3bb'>Sequence and Features</span>
−	<partinfo>BBa_K3196025 SequenceAndFeatures</partinfo>
		+	<h1>'''Reference'''</h1><!-- -->
		+	[1]Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzyme biochemical properties. Nooshin Mohandesi,  Seyed Omid Ranaei Siadat, Kamahldin Haghbeen, Ardeshir Hesampour Received: 8 February 2016 / Accepted: 25 May 2016 / Published online: 8 June 2016 The Author(s) 2016.
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		+	<partinfo>BBa_K3196025 SequenceAndFeatures</partinfo>
	<!-- Uncomment this to enable Functional Parameter display		<!-- Uncomment this to enable Functional Parameter display

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Latest revision as of 10:40, 20 October 2019

AOX1-Kozak-SUC2-VP-His tag-AOX1 Terminator

The SUC2 gene of S. cerevisiae, encoding invertase was designed to codon preference of P. pastoris excluding the sequence of the N-terminal pre-pro-sequence signal peptide and His-tag in the C terminus. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.The expression of SUC2 gene is a kind of Sucrose transferase [1], and its signal peptide can help the protein out cells. We use this signal peptide raise the efficiency of VP’s transformation.

Reference

[1]Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzyme biochemical properties. Nooshin Mohandesi, Seyed Omid Ranaei Siadat, Kamahldin Haghbeen, Ardeshir Hesampour Received: 8 February 2016 / Accepted: 25 May 2016 / Published online: 8 June 2016 The Author(s) 2016.