Difference between revisions of "Part:BBa K3196024"
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<partinfo>BBa_K3196024 short</partinfo> | <partinfo>BBa_K3196024 short</partinfo> | ||
− | PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze | + | PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin. |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.[[File:T--HUST--China--2019-PHO1-αproVP.jpg|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1-αproVP]] | This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.[[File:T--HUST--China--2019-PHO1-αproVP.jpg|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1-αproVP]] | ||
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Latest revision as of 10:40, 20 October 2019
AOX1-Kozak-PHO1 pro-VP-His tag-AOX1 Terminator
PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin.
Characterization
This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 937
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]