Revision as of 11:49, 19 October 2019 (view source) Satirev (Talk | contribs) ← Older edit		Latest revision as of 10:40, 20 October 2019 (view source) Satirev (Talk | contribs)
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	<partinfo>BBa_K3196024 short</partinfo>		<partinfo>BBa_K3196024 short</partinfo>
−	PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze pectin.	+	PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin.
	<h1>'''Characterization'''</h1>		<h1>'''Characterization'''</h1>
−	This is a four section for degrade and transfer lignin part.	+	This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.[[File:T--HUST--China--2019-PHO1-αproVP.jpg‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1-αproVP]]
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]	+
−	<h1>'''DNA Gel Electrophoretic'''</h1>
−	After we link PHO1 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
−
−	<h1>'''SDS-PAGE'''</h1>
−	We run the SDS-PAGE to check whether PHO1 αpro help the enzyme transfer to the extracellular. As the figure shows, we get the protein type about xxx bp which means the SDS-PAGE is successful.
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
−
−	<h1>'''Enzyme Activity'''</h1>
−	We use manganese and hydrogen peroxide to detect the enzyme activity. As the figure shows, the solution turns xxx, which confirm the enzyme activity.
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
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Latest revision as of 10:40, 20 October 2019

AOX1-Kozak-PHO1 pro-VP-His tag-AOX1 Terminator

PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.

Sequence and Features