Difference between revisions of "Part:BBa K3196024"

 
 
(4 intermediate revisions by 3 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3196024 short</partinfo>
 
<partinfo>BBa_K3196024 short</partinfo>
  
protein
+
PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin.
 +
 
 +
<h1>'''Characterization'''</h1>
 +
This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.[[File:T--HUST--China--2019-PHO1-αproVP.jpg‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1-αproVP]]
  
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->

Latest revision as of 10:40, 20 October 2019


AOX1-Kozak-PHO1 pro-VP-His tag-AOX1 Terminator

PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.
Figure1. T--HUST--China--2019-PHO1-αproVP


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]