Latest revision as of 10:40, 20 October 2019

AOX1-Kozak-PHO1-VP-His tag-AOX1 Terminator

PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.PHO1 is a widely used signal peptide in eukaryotic expression system. It can help the peptide chain of VP be transferred out cells.

Figure1. T--HUST--China--2019-PHO1VP

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 937
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3196023 short</partinfo>
-PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. VP can catalyze pectin.
+PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. VP can catalyze lignin.
 <h1>'''Characterization'''</h1>
 This is a four section for degrade and transfer lignin part.PHO1 is a widely used signal peptide in eukaryotic expression system. It can help the peptide chain of VP be transferred out cells.[[File:T--HUST--China--2019-PHO1VP.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1VP]]
-<h1>'''DNA Gel Electrophoretic'''</h1>
-After we link PHO1 and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
-[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]

Difference between revisions of "Part:BBa K3196023"

Latest revision as of 10:40, 20 October 2019

Characterization