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	<partinfo>BBa_K3196023 short</partinfo>		<partinfo>BBa_K3196023 short</partinfo>
−	PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. VP can catalyze pectin.	+	PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. VP can catalyze lignin.
	<h1>'''Characterization'''</h1>		<h1>'''Characterization'''</h1>
−	This is a four section for degrade and transfer lignin part.	+	This is a four section for degrade and transfer lignin part.PHO1 is a widely used signal peptide in eukaryotic expression system. It can help the peptide chain of VP be transferred out cells.[[File:T--HUST--China--2019-PHO1VP.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1VP]]
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]	+
−	<h1>'''DNA Gel Electrophoretic'''</h1>
−	After we link PHO1 and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
−	<h1>'''SDS-PAGE'''</h1>
−	We run the SDS-PAGE to check whether PHO1 help the enzyme transfer to the extracellular. As the figure shows, we get the protein type about xxx bp which means the SDS-PAGE is successful.
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
−
−	<h1>'''Enzyme Activity'''</h1>
−	We use manganese and hydrogen peroxide to detect the enzyme activity. As the figure shows, the solution turns xxx, which confirm the enzyme activity.
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
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Latest revision as of 10:40, 20 October 2019

AOX1-Kozak-PHO1-VP-His tag-AOX1 Terminator

PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.PHO1 is a widely used signal peptide in eukaryotic expression system. It can help the peptide chain of VP be transferred out cells.

Sequence and Features