Revision as of 11:44, 19 October 2019 (view source) Satirev (Talk | contribs) ← Older edit		Latest revision as of 10:39, 20 October 2019 (view source) Satirev (Talk | contribs)
(4 intermediate revisions by 3 users not shown)
Line 3:		Line 3:
	<partinfo>BBa_K3196022 short</partinfo>		<partinfo>BBa_K3196022 short</partinfo>
−	FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze pectin.	+	FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze lignin.
	<h1>'''Characterization'''</h1>		<h1>'''Characterization'''</h1>
−	This is a four section for degrade and transfer lignin part.	+	This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.<sup>[1]</sup> It can help the peptide chain of VP be transferred out cells. [[File:T--HUST--China--2019-FLO10-αproVP.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-FLO10-αproVP]]
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]	+
−	<h1>'''DNA Gel Electrophoretic'''</h1>	+	<h1>'''Reference'''</h1>
−	After we link FLO10 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.	+	[1]Effect of signal peptides on the expression of laccase in Pichia pastoris  Shi Yiping , Zhou Xue , Hu Meirong , Lin Jianhui , Tao Yong , Huang Jianzhong  Acta Microbiologica Sinica 54(12) :1446 － 1452; 4 December 2014 ISSN 0001 － 6209; CN 11 － 1995 /Q
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]	+
−		+
−	<h1>'''SDS-PAGE'''</h1>	+
−	We run the SDS-PAGE to check whether FLO10 αpro help the enzyme transfer to the extracellular. As the figure shows, we get the protein type about xxx bp which means the SDS-PAGE is successful.	+
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]	+
−		+
−	<h1>'''Enzyme Activity'''</h1>	+
−	We use manganese and hydrogen peroxide to detect the enzyme activity. As the figure shows, the solution turns xxx, which confirm the enzyme activity.	+
−	[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]	+
	<!-- -->		<!-- -->

---

Latest revision as of 10:39, 20 October 2019

AOX1-Kozak-FLO10 pro-VP-His tag-AOX1 Terminator

FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.^[1] It can help the peptide chain of VP be transferred out cells.

Reference

[1]Effect of signal peptides on the expression of laccase in Pichia pastoris Shi Yiping , Zhou Xue , Hu Meirong , Lin Jianhui , Tao Yong , Huang Jianzhong Acta Microbiologica Sinica 54(12) :1446 － 1452; 4 December 2014 ISSN 0001 － 6209; CN 11 － 1995 /Q

Sequence and Features