Difference between revisions of "Part:BBa K3196021"
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<partinfo>BBa_K3196021 short</partinfo> | <partinfo>BBa_K3196021 short</partinfo> | ||
| − | protein | + | FLO10 is a Lectin-like protein with similarity to Flo1 p, thought to be expressed and involved in flocculation. VP can catalyze lignin. |
| − | < | + | <h1>'''Characterization'''</h1> |
| − | + | This is a four section for degrade and transfer lignin part.FLO10 is a kind of signal peptide which was initially found before the protein involved in flocculation. It is supposed that the signal peptides from pichia pastoris can be better identified by its signal recognition system, thus benefiting the secretion of exogenous protein. So we add the coding sequence of FLO10 before VP to figure out whether it could increase the output of VP. | |
| + | [[File:T--HUST--China--2019-FLO10VP.jpg |400px|thumb|center|Figure1. T--HUST--China--2019-FLO10VP]] | ||
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Latest revision as of 10:36, 20 October 2019
AOX1-Kozak-FLO10-VP-His tag-AOX1 Terminator
FLO10 is a Lectin-like protein with similarity to Flo1 p, thought to be expressed and involved in flocculation. VP can catalyze lignin.
Characterization
This is a four section for degrade and transfer lignin part.FLO10 is a kind of signal peptide which was initially found before the protein involved in flocculation. It is supposed that the signal peptides from pichia pastoris can be better identified by its signal recognition system, thus benefiting the secretion of exogenous protein. So we add the coding sequence of FLO10 before VP to figure out whether it could increase the output of VP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 937
Illegal BamHI site found at 948 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]