Difference between revisions of "Part:BBa K2963003"

Latest revision as of 10:01, 20 October 2019

capB* - a subunit of poly-γ-glutamic acid synthetase

This part contains the capB gene which encodes a subunit for poly-γ-glutamic acid synthetase. This enzyme is involved in poly-γ-glutamic acid synthesis, which converts the glutamate to poly-γ-glutamic acid. CapB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we used the mutant of capB gene which calls capB*.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 737
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 737
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 737
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 737
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 737
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2963003 short</partinfo>
-This part contains the pgsB gene for poly-gamma-glutamic acid synthetase. This enzyme is involved in poly-gamma-glutamic synthesis,which converts the glutamate to poly-gamma-glutamic acid. PgsB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we get the pgsB sequenece by error-prone PCR of Bacillus genome, trying to improve the activity of PgsB protein.
+This part contains the <i>capB</i> gene which encodes a subunit for poly-γ-glutamic acid synthetase. This enzyme is involved in poly-γ-glutamic acid synthesis, which converts the glutamate to poly-γ-glutamic acid. CapB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we used the mutant of <i>capB</i> gene which calls <i>capB*</i>.
 <!-- Add more about the biology of this part here