Difference between revisions of "Part:BBa K2913021"

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We added gradient of 5-FC culturing the bacteria and monitor their growth.
 
We added gradient of 5-FC culturing the bacteria and monitor their growth.
  
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[[Image:T--NEFU_China--parts--CD1.png|600px|thumb|right|Fig.1 Growth of E.coli at different concentration of 5-FC. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging : 2μl bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB mediacontaining 50µg/ml Amp and str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria (E.coli) using T7 to express CD. B. The bacteria (E.coli) can`t express CD.]]
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[[Image:T--NEFU_China--parts--CD1.png|600px|thumb|left|Fig.1 Growth of E.coli at different concentration of 5-FC. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging : 2μl bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB mediacontaining 50µg/ml Amp and str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria (E.coli) using T7 to express CD. B. The bacteria (E.coli) can`t express CD.]]
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As a positive control, we added gradient of 5-FU culturing the bacteria and monitor their growth.
  
 
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Revision as of 09:35, 20 October 2019


Cytosine deaminase

An enzyme that oxidizes 5-fluorouracil to 5-fluorouracil

Usage and Biology

result

Cytosine deaminase (CD) is the enzyme that can transform cytosine into uracil. CD’s function is to convert nontoxic 5-Flucytosine (5-FC) into toxic 5-fluorouracil 5-FU, which has an extreme damage to the bacteria.

We added gradient of 5-FC culturing the bacteria and monitor their growth.

Fig.1 Growth of E.coli at different concentration of 5-FC. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging : 2μl bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB mediacontaining 50µg/ml Amp and str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria (E.coli) using T7 to express CD. B. The bacteria (E.coli) can`t express CD.

As a positive control, we added gradient of 5-FU culturing the bacteria and monitor their growth.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1140
  • 1000
    COMPATIBLE WITH RFC[1000]