Difference between revisions of "Part:BBa K3196019"
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<h1>'''DNA Gel Electrophoretic'''</h1>
 
<h1>'''DNA Gel Electrophoretic'''</h1>
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After we link PHO1 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1100 bp which means the PCR is successful.
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After we link PHO1 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1092 bp which means the PCR is successful.
 
[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 
[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
  

Revision as of 08:47, 20 October 2019


AOX1-Kozak-PHO1 pro-pelA-His tag-AOX1 Terminator

PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze pectin.

Characterization

This is a four section for degrade and transfer pectin part.

Figure1. T--HUST--China--2019-PHO1-αpropelA

DNA Gel Electrophoretic

After we link PHO1 αpro and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1092 bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1968
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]