Difference between revisions of "Part:BBa K3126022"

Revision as of 08:04, 20 October 2019

nixA-TgMTP1t2-KanR

We connected TgMTP1t2 and nixA with their promoters and terminators, and used KanR as resistance cassette. We characterized the ability to adsorb nickel ions of Saccharomyces cerevisiae which expressed this part. We also compared its function with those two functional genes working separately (Part:BBa_K3126020 Part:BBa_K3126019).

Usage and Biology

Here is the curve comparing with Saccharomyces cerevisiae expressing Part:BBa_K3126020 and Part:BBa_K3126019. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .

Engineering yeast comparing with BBa_K3126020 and BBa_K3126019

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2574
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4048
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3126022 short</partinfo>
-Can remove nickel ions from water.
+We connected TgMTP1t2 and nixA with their promoters and terminators, and used KanR as resistance cassette. We characterized the ability to adsorb nickel ions of <em><span>Saccharomyces cerevisiae</span></em> which expressed this part.  We also compared its function with those two functional genes working separately ([[Part:BBa_K3126020]] [[Part:BBa_K3126019]]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Here is the curve comparing with <em><span>Saccharomyces cerevisiae</span></em> expressing [[Part:BBa_K3126020]] and [[Part:BBa_K3126019]]. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .
+[[File:Ni-tg-nitg.png|500px|thumb|Engineering yeast comparing with BBa_K3126020 and BBa_K3126019]]
 <!-- -->