Difference between revisions of "Part:BBa K3196018"
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<partinfo>BBa_K3196018 short</partinfo> | <partinfo>BBa_K3196018 short</partinfo> | ||
| − | PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. pelA can | + | PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. pelA can catalyze pectin. |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
Revision as of 06:54, 20 October 2019
AOX1-Kozak-PHO1-pelA-His tag-AOX1 Terminator
PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. pelA can catalyze pectin.
Characterization
This is a four section for degrade and transfer pectin part.
DNA Gel Electrophoretic
After we link PHO1 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about 1100 bp which means the PCR is successful.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1767
Illegal BamHI site found at 937 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]