Difference between revisions of "Part:BBa K2922029"
Line 4: Line 4:
 
===Summary===
 
===Summary===
 
Abbreviated as Nkil, lysis protein for Colicin-N is a 5.6 kDa protein, which is encoded by gene <i>cnl</i>. The lysis protein is required for both Colicin-E1 release and partial cell lysis[1]. (Fig.1)
 
Abbreviated as Nkil, lysis protein for Colicin-N is a 5.6 kDa protein, which is encoded by gene <i>cnl</i>. The lysis protein is required for both Colicin-E1 release and partial cell lysis[1]. (Fig.1)
−
<table><tr><th>[[File:Ndesign.png|thumb|300px|Fig.1 Nkil helps Colicin-N release and makes partial cell lysis.]]</th><th></table>
+
<table><tr><th>[[File:Ndesign.png|thumb|720px|Fig.1 Nkil helps Colicin-N release and makes partial cell lysis.]]</th><th></table>
 
===Identification===
 
===Identification===
−
When we are building this circuit, we are doing the nucleic acid gel electrophoresis experiment to verify. After the circuit is built, we send the plasmid to sequence, and get the correct sequencing.
+
When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit is built, we sent the plasmid to sequence, and got the correct sequencing.
−
After new molecular cloning experiments, we do Enzyme-Cut identification to certify the plasmid is correct. We use the <i>Xba</i>I and <i>Pst</i>I to cut the plasmid, then we get the target separate fragment-159bp. (Fig.2)
+
After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid was correct. We used the <i>Xba</i>I and <i>Pst</i>I to cut the plasmid, then we got the target separate fragment-159bp. (Fig.2)
−
<table><tr><th>[[File:Nkil-Ekil.png|thumb|300px|Fig.2 The result of this plasmid cut with enzyme <i>Xba</i>I and <i>Pst</i>I.]]</th><th></table>
+
<table><tr><th>[[File:Nkil-Ekil.png|thumb|720px|Fig.2 The result of this plasmid cut with enzyme <i>Xba</i>I and <i>Pst</i>I.]]</th><th></table>
 
===Reference===
 
===Reference===
 
[1] A. P. Pugsley, The immunity and lysis genes of ColN plasmid pCHAP4. Molecular & General Genetics Mgg 211, 335-341 (1988).
 
[1] A. P. Pugsley, The immunity and lysis genes of ColN plasmid pCHAP4. Molecular & General Genetics Mgg 211, 335-341 (1988).

Revision as of 02:53, 20 October 2019


Lysis protein for colicin-N coding region

Summary

Abbreviated as Nkil, lysis protein for Colicin-N is a 5.6 kDa protein, which is encoded by gene cnl. The lysis protein is required for both Colicin-E1 release and partial cell lysis[1]. (Fig.1)

Fig.1 Nkil helps Colicin-N release and makes partial cell lysis.

Identification

When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit is built, we sent the plasmid to sequence, and got the correct sequencing. After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid was correct. We used the XbaI and PstI to cut the plasmid, then we got the target separate fragment-159bp. (Fig.2)

Fig.2 The result of this plasmid cut with enzyme XbaI and PstI.

Reference

[1] A. P. Pugsley, The immunity and lysis genes of ColN plasmid pCHAP4. Molecular & General Genetics Mgg 211, 335-341 (1988).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 118
  • 1000
    COMPATIBLE WITH RFC[1000]