Difference between revisions of "Part:BBa K3254022"

Latest revision as of 02:10, 20 October 2019

lacI constitutive expression cassette

This part can express LacI protein constitutively. It can be used on regulating the promoters with lacO operators, such as Ptac(BBa_K864400) part and the derived Ptac promoter,such as BBa_K2572025,BBa_K3254015,BBa_K3254016 and BBa_K864407.

Usage and Biology

The functionalities of the inducible divice made up by this part and Ptac derived promoters on a P15A plasmid in E.coli DH5α host cell were described on the page of BBa_K3254025.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3254022 short</partinfo>
-This part can express LacI protein constitutively. It can be used on regulating the promoters with lacO operators, such as Ptac([[Part:BBa_K864400|BBa_K864400]]) part and the derived Ptac promoter,such as [[Part:BBa_K3254014|BBa_K3254014]],[[Part:BBa_K3254015|BBa_K3254015]],[[Part:BBa_K3254016|BBa_K3254016]] and [[Part:BBa_K3254017|BBa_K864407]].
+This part can express LacI protein constitutively. It can be used on regulating the promoters with lacO operators, such as Ptac([[Part:BBa_K864400|BBa_K864400]]) part and the derived Ptac promoter,such as [[Part:BBa_K2572025|BBa_K2572025]],[[Part:BBa_K3254015|BBa_K3254015]],[[Part:BBa_K3254016|BBa_K3254016]] and [[Part:BBa_K3254017|BBa_K864407]].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The functionalities of the inducible divice made up by this part and Ptac derived promoters on a P15A plasmid in E.coli DH5α host cell were described on the page of [[Part:BBa_K3254025|BBa_K3254025]].
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3254022 SequenceAndFeatures</partinfo>