Difference between revisions of "Part:BBa K3254022"
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<partinfo>BBa_K3254022 short</partinfo> | <partinfo>BBa_K3254022 short</partinfo> | ||
| − | This part can express LacI protein constitutively. It can be used on regulating the promoters with lacO operators, | + | This part can express LacI protein constitutively. It can be used on regulating the promoters with lacO operators, such as Ptac([[Part:BBa_K864400|BBa_K864400]]) part and the derived Ptac promoter,such as [[Part:BBa_K2572025|BBa_K2572025]],[[Part:BBa_K3254015|BBa_K3254015]],[[Part:BBa_K3254016|BBa_K3254016]] and [[Part:BBa_K3254017|BBa_K864407]]. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The functionalities of the inducible divice made up by this part and Ptac derived promoters on a P15A plasmid in E.coli DH5α host cell were described on the page of [[Part:BBa_K3254025|BBa_K3254025]]. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3254022 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3254022 SequenceAndFeatures</partinfo> | ||
Latest revision as of 02:10, 20 October 2019
lacI constitutive expression cassette
This part can express LacI protein constitutively. It can be used on regulating the promoters with lacO operators, such as Ptac(BBa_K864400) part and the derived Ptac promoter,such as BBa_K2572025,BBa_K3254015,BBa_K3254016 and BBa_K864407.
Usage and Biology
The functionalities of the inducible divice made up by this part and Ptac derived promoters on a P15A plasmid in E.coli DH5α host cell were described on the page of BBa_K3254025.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]