Difference between revisions of "Part:BBa K2957035"
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What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid. | What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid. | ||
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| + | If you would like the complete correct sequence please contact igem-2019-students@mit.edu and igem-2019-mentors@mit.edu | ||
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Latest revision as of 02:08, 20 October 2019
TRE-tight, mKate
Contents: TRE, mKate2, SynthpA, with Amp resistance
What is it?: A Composite part; it is a genetic circuit with TRE-tight promoter and mKate2 reporter gene.
What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid.
If you would like the complete correct sequence please contact igem-2019-students@mit.edu and igem-2019-mentors@mit.edu
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30
Illegal XbaI site found at 1051 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30
Illegal XbaI site found at 1051 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30
Illegal XbaI site found at 1051
Illegal AgeI site found at 1057 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 784
Illegal BsaI.rc site found at 973