Difference between revisions of "Part:BBa K2957035"
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2957035 short</partinfo>
 
<partinfo>BBa_K2957035 short</partinfo>
  
−
Contents: TRE, IL-8 (CXCL-8), NeonGreen tag, SynthpA, with Carb resistance  
+
Contents: TRE, mKate2, SynthpA, with Amp resistance  
  
−
What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for inducible IL-8 expression.
+
What is it?: A Composite part; it is a genetic circuit with TRE-tight promoter and mKate2 reporter gene.
  
−
What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It has a NeonGreen tag attached.  
+
What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid.  
  
−
How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL-8. See results below + see more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
+
If you would like the complete correct sequence please contact igem-2019-students@mit.edu and igem-2019-mentors@mit.edu
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:08, 20 October 2019


TRE-tight, mKate

Contents: TRE, mKate2, SynthpA, with Amp resistance

What is it?: A Composite part; it is a genetic circuit with TRE-tight promoter and mKate2 reporter gene.

What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid.

If you would like the complete correct sequence please contact igem-2019-students@mit.edu and igem-2019-mentors@mit.edu

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1051
    Illegal AgeI site found at 1057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 784
    Illegal BsaI.rc site found at 973