Difference between revisions of "Part:BBa K3179100"

Revision as of 01:10, 20 October 2019

pTREK

This part consists of Vector pTRE, miR663b-target, 2kt-EGFP and miR885-5p-target. There is a CMV promoter on pTRE that can transcribe mRNAs containing miR663b-target, 2kt-EGFP and miR885-5p-target. The translation of this mRNA is regulated by L7Ae and the amount of intracellular free miRNA663b and miRNA885-5p. When miRNA663b, miRNA885-5p and L7Ae exist, the translation of this mRNA is suppressed. The mRNA’s translation product is EGFP, and green fluorescence can be observed to facilitate observation of gene expression results.

In our design, pTRE-miR663b-2kt-EGFP-miR885-5p is used for co-transduction with pTRE-L7Ae-miR592, and the compatibility and effectiveness of L7Ae-Kturn system and Tet-On system can be tested by measuring the expression amount of EGFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 195
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 201
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 857

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3179100 short</partinfo>
-This part is consisted of Vector pTRE, miR663b-target, 2kt-EGFP and miR885-5p-target. There is a CMV promoter on pTRE that can transcribe mRNAs with miR663b-target, 2kt-EGFP and miR885-5p-target. The translation of this mRNA is controlled by L7Ae and the amount of intracellular free miRNA663b and miRNA885-5p. When miRNA663b, miRNA885-5p and L7Ae exist, the translation of this mRNA is suppressed. The mRNA’s translation product is EGFP, and green fluorescence can be observed to facilitate observation of gene expression results.
+This part consists of Vector pTRE, miR663b-target, 2kt-EGFP and miR885-5p-target. There is a CMV promoter on pTRE that can transcribe mRNAs containing miR663b-target, 2kt-EGFP and miR885-5p-target. The translation of this mRNA is regulated by L7Ae and the amount of intracellular free miRNA663b and miRNA885-5p. When miRNA663b, miRNA885-5p and L7Ae exist, the translation of this mRNA is suppressed. The mRNA’s translation product is EGFP, and green fluorescence can be observed to facilitate observation of gene expression results.
 In our design, pTRE-miR663b-2kt-EGFP-miR885-5p is used for co-transduction with pTRE-L7Ae-miR592, and the compatibility and effectiveness of L7Ae-Kturn system and Tet-On system can be tested by measuring the expression amount of EGFP.