Difference between revisions of "Part:BBa K3179003"

Revision as of 00:37, 20 October 2019

miR592-target

miR592-target is constructed bearing four repeats of miRNA 592, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.
This part, four repeating sequences complementary to miRNA592 on an mRNA, is a 4X miRNA target designed to target miRNA592. When miRNA592 reaches a certain concentration in the cell, it would combine with miR592-target and suppress the translation of mRNA. According to our design, miR592-target is designed to regulate the translation of mRNA with response parts. We integrate miR592-target with L7Ae so that L7Ae cannot be translated with miRNA592 existing in the cell.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 miR592-target is constructed bearing four repeats of miRNA 592, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.
-<br style="clear: both" />This part, four repeating sequences complementary to miRNA592 on an mRNA, is a 4X miRNA target designed to target miRNA592. When miRNA592 reaches a certain concentration in the cell, it would combine with miR592-target and suppress the translation of mRNA. According to our design, miR592-target is designed to control the translation of mRNA with response parts. We integrate miR592-target with L7Ae so that L7Ae cannot be translated with miRNA592 existing in the cell.
+<br style="clear: both" />This part, four repeating sequences complementary to miRNA592 on an mRNA, is a 4X miRNA target designed to target miRNA592. When miRNA592 reaches a certain concentration in the cell, it would combine with miR592-target and suppress the translation of mRNA. According to our design, miR592-target is designed to regulate the translation of mRNA with response parts. We integrate miR592-target with L7Ae so that L7Ae cannot be translated with miRNA592 existing in the cell.
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