Difference between revisions of "Part:BBa K2957013"
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<partinfo>BBa_K2957013 short</partinfo> | <partinfo>BBa_K2957013 short</partinfo> | ||
| − | + | <b>Contents:</b> CCL5 (RANTES), NeonGreen tag, with Carb resistance | |
| − | What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly) | + | <b>What is it?:</b> A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly) |
| − | What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a NeonGreen tag attached. | + | <b>What does it do?:</b> It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a NeonGreen tag attached. |
| − | How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>." | + | <b>How to use it?:</b> You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>." |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 23:47, 19 October 2019
CCL5 with NeonGreen tag (C-terminal)
Contents: CCL5 (RANTES), NeonGreen tag, with Carb resistance
What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)
What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a NeonGreen tag attached.
How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 442
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 442
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 458
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 442
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 442
- 1000COMPATIBLE WITH RFC[1000]