Difference between revisions of "Part:BBa K2957004"

Latest revision as of 23:37, 19 October 2019

CMV, IL-8 (CXCL-8)

Contents: CMV, IL-8 (CXCL-8), SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See results below + see more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1113
Illegal SpeI site found at 92
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 92
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1031
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1113
Illegal SpeI site found at 92
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1113
Illegal SpeI site found at 92
Illegal AgeI site found at 1119
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2957004 short</partinfo>
-Contents: CMV, IL-8 (CXCL-8), SynthpA, with Carb resistance
+<b>Contents:</b> CMV, IL-8 (CXCL-8), SynthpA, with Carb resistance
-What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression
+<b>What is it?:</b> A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression
-What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its
+<b>What does it do?:</b> It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its
 involvement in the immune system and is known to create neutrophil swarming.
-How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See results below + see more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
+<b>How to use it?:</b> This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See results below + see more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
 <!-- Add more about the biology of this part here