Difference between revisions of "Part:BBa K2984010"
 
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Plasmid backbone for MoClo L0 cloning with an RFP insert for selection.  
 
This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Plasmid backbone for MoClo L0 cloning with an RFP insert for selection.  
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===Usage and Biology===
 
  
 
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==Characterization==
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===Functional Parameters===
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<partinfo>BBa_K2984010 parameters</partinfo>
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<img src="https://2019.igem.org/wiki/images/3/3a/T--Humboldt_Berlin--notebook_s_1203.png" alt="L0-RFP-clone" width="500" />
 
<img src="https://2019.igem.org/wiki/images/3/3a/T--Humboldt_Berlin--notebook_s_1203.png" alt="L0-RFP-clone" width="500" />
 
<figcaption>Fig. 1 - Our self-constructed L0-RFP backbone transformed into <i>E. coli</i> (strain DH10B). Through red-/white-selection successful clones were picked and streaked out on LB-agar plates.</figcaption>
 
<figcaption>Fig. 1 - Our self-constructed L0-RFP backbone transformed into <i>E. coli</i> (strain DH10B). Through red-/white-selection successful clones were picked and streaked out on LB-agar plates.</figcaption>
  
 
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===Usage and Biology===
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===Functional Parameters===
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<partinfo>BBa_K2984010 parameters</partinfo>
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Latest revision as of 23:32, 19 October 2019


L0 Backbone

This vector is a part of the Chlamy-HUB-Collection. Plasmid backbone for MoClo L0 cloning with an RFP insert for selection.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2860
    Illegal AgeI site found at 1014
    Illegal AgeI site found at 1126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 829
    Illegal BsaI.rc site found at 1918


Characterization

L0-RFP-clone

Fig. 1 - Our self-constructed L0-RFP backbone transformed into E. coli (strain DH10B). Through red-/white-selection successful clones were picked and streaked out on LB-agar plates.