Difference between revisions of "Part:BBa K3183201"
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This part contains CD27L fused to an N-Terminal 6-His Tag and SpyTag for purification, oligomerisation, and other SpyCatcher applications. This endolysin has previously been shown to be specific to <i>C. difficile</i>. This endolysin works by cleaving residues<sup>1</sup> (Fig. 1), breaking the cell's peptidoglycan and compromising the structural integrity of its cell wall. Two X-ray crystal structures were solved for the N<sup>2</sup> and C-terminal domains<sup>3</sup>. These are joined by a linker, which we've added ourselves (Figure 2).
 
This part contains CD27L fused to an N-Terminal 6-His Tag and SpyTag for purification, oligomerisation, and other SpyCatcher applications. This endolysin has previously been shown to be specific to <i>C. difficile</i>. This endolysin works by cleaving residues<sup>1</sup> (Fig. 1), breaking the cell's peptidoglycan and compromising the structural integrity of its cell wall. Two X-ray crystal structures were solved for the N<sup>2</sup> and C-terminal domains<sup>3</sup>. These are joined by a linker, which we've added ourselves (Figure 2).
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[[File:BBa_K3183009_Structure.png|thumb|right|430px|'''Figure 2:''' X-ray Crystal Structure of CD27L (PDB codes 3QAY & 4CU5]]
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[[File:BBa K3183012 Reaction.png|thumb|right|430px|'''Figure 2:''' X-ray Crystal Structure of CD27L (PDB codes 3QAY & 4CU5]]
 
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[[File:BBa K3183012 Reaction.png|thumb|left|430px|'''Figure 1:''' CD27L Catalytic Reaction.]]
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[[File:BBa_K3183009_Reaction.png|thumb|left|430px|'''Figure 1:''' CD27L Catalytic Reaction.]]
 
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Revision as of 23:27, 19 October 2019


Truncated CD27L 1-179 with 6-His and SpyTag

This part contains CD27L fused to an N-Terminal 6-His Tag and SpyTag for purification, oligomerisation, and other SpyCatcher applications. This endolysin has previously been shown to be specific to C. difficile. This endolysin works by cleaving residues1 (Fig. 1), breaking the cell's peptidoglycan and compromising the structural integrity of its cell wall. Two X-ray crystal structures were solved for the N2 and C-terminal domains3. These are joined by a linker, which we've added ourselves (Figure 2).

Figure 2: X-ray Crystal Structure of CD27L (PDB codes 3QAY & 4CU5





Figure 1: CD27L Catalytic Reaction.












Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]