Difference between revisions of "Part:BBa K3183201"

Revision as of 23:25, 19 October 2019

Truncated CD27L 1-179 with 6-His and SpyTag

This part contains CD27L fused to an N-Terminal 6-His Tag and SpyTag for purification, oligomerisation, and other SpyCatcher applications. This endolysin has previously been shown to be specific to C. difficile. This endolysin works by cleaving residues¹ (Fig. 1), breaking the cell's peptidoglycan and compromising the structural integrity of its cell wall. Two X-ray crystal structures were solved for the N² and C-terminal domains³. These are joined by a linker, which we've added ourselves (Figure 2).

Figure 2: X-ray Crystal Structure of CD27L (PDB codes 3QAY & 4CU5

Figure 1: CD27L Catalytic Reaction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K3183201 short</partinfo>
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 This part contains CD27L fused to an N-Terminal 6-His Tag and SpyTag for purification, oligomerisation, and other SpyCatcher applications. This endolysin has previously been shown to be specific to <i>C. difficile</i>. This endolysin works by cleaving residues<sup>1</sup> (Fig. 1), breaking the cell's peptidoglycan and compromising the structural integrity of its cell wall. Two X-ray crystal structures were solved for the N<sup>2</sup> and C-terminal domains<sup>3</sup>. These are joined by a linker, which we've added ourselves (Figure 2).