Difference between revisions of "Part:BBa K3105681:Design"

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===Design Notes===
 
===Design Notes===
−
For this design, overhangs complementary to the pPICZ&#945;B vector (Invitrogen easy select <I>Pichia</I> expression kit) were added by PCR. This was then assembled into the expression vector generating the circuit in this part
+
For this design, overhangs complementary to the pPICZ&#945;B vector (Invitrogen easy select <I>Pichia</I> expression kit) were added to <partinfo>BBa_K500000</partinfo> by PCR. This was then assembled into the expression vector generating the circuit in this part
  
 
===Source===
 
===Source===

Latest revision as of 23:23, 19 October 2019


Expression construct LiP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2453
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For this design, overhangs complementary to the pPICZαB vector (Invitrogen easy select Pichia expression kit) were added to BBa_K500000 by PCR. This was then assembled into the expression vector generating the circuit in this part

Source

see basic parts

References