Difference between revisions of "Part:BBa K2957008"
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<partinfo>BBa_K2957008 short</partinfo> | <partinfo>BBa_K2957008 short</partinfo> | ||
| − | Contents: | + | Contents: CMV, IL-8 (CXCL-8), SynthpA, with Carb resistance |
What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression | What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression | ||
What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. | What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. | ||
Revision as of 23:23, 19 October 2019
CMV, IL-8 with NeonGreen Tag (C-terminal)
Contents: CMV, IL-8 (CXCL-8), SynthpA, with Carb resistance What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts>.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1802
Illegal SpeI site found at 92
Illegal PstI site found at 1245 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 92
Illegal PstI site found at 1245 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1019
Illegal BamHI site found at 1261 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1802
Illegal SpeI site found at 92
Illegal PstI site found at 1245 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1802
Illegal SpeI site found at 92
Illegal PstI site found at 1245
Illegal AgeI site found at 1808 - 1000COMPATIBLE WITH RFC[1000]