Difference between revisions of "Part:BBa K3105680:Design"
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===Design Notes=== | ===Design Notes=== | ||
Sequence was codon optimised for <I>Pichia pastoris</I> <br> | Sequence was codon optimised for <I>Pichia pastoris</I> <br> | ||
| − | eGFP was added to the C-terminal | + | eGFP was added to the C-terminal to identify AAO-expressing <I>P. patoris</I> clones. |
===Source=== | ===Source=== | ||
Latest revision as of 23:20, 19 October 2019
Expression construct AAO-2A-eGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1356
Illegal BamHI site found at 1543
Illegal XhoI site found at 1183 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2503
Illegal AgeI site found at 3896 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was codon optimised for Pichia pastoris
eGFP was added to the C-terminal to identify AAO-expressing P. patoris clones.
Source
see basic parts