Difference between revisions of "Part:BBa K2984071"

 
 
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<partinfo>BBa_K2984071 short</partinfo>
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One restricting factor for cultivating microalgae is the high risk of contamination with bacteria, fungi or zooplankton. As our Team anticipates in building a bioreactor it is essential to sterilize the media and all containers which raise the costs of production. Loera-Quezada et al. (2016) found a way to bypass this problem by integrating <i>Pseudomonas stutzeri WM88</i> gene <i>ptxD</i> in <i>C. reinhardtii</i>. It encodes a phosphite oxidoreductase which is able to oxidize phosphite into phosphate using NAD+ as a cofactor (White & Metcalf, 2007). The integration of <i>ptxD</i> into the genome of <i>C. reinhardtii</i> enables the use of phosphite as a sole phosphorus source which wasn’t possible before (Loera-Quezada et al., 2015). Loera-Quezada et al. (2016) presented a new way of microalgae production without the need for sterile conditions or antibiotics to avoid contamination with biological pollutants as they cannot use phosphite as a phosphorus source. In the future, this system could also be applied to open pond systems.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2984071 SequenceAndFeatures</partinfo>
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===MoClo Overhangs===
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<p>This part with B3-B4 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984013">here</a>.</p>
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<p>This part with B3-B3 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984026">here</a>.
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</p>
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</html>
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===Functional Parameters===
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<partinfo>BBa_K2984067 parameters</partinfo>
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==References==
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<ol>
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Loera‐Quezada, M. M., Leyva‐González, M. A., Velázquez‐Juárez, G., Sanchez‐Calderón, L., Do Nascimento, M., López‐Arredondo, D., & Herrera‐Estrella, L. (2016). A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae. Plant biotechnology journal, 14(10), 2066-2076.
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</li>
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<li>
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White, A. K., & Metcalf, W. W. (2007). Microbial metabolism of reduced phosphorus compounds. Annu. Rev. Microbiol., 61, 379-400.
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</li>
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<li>
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Loera-Quezada, M. M., Leyva-González, M. A., López-Arredondo, D., & Herrera-Estrella, L. (2015). Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae. Plant Science, 231, 124-130.
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</li>
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</ol>

Latest revision as of 23:20, 19 October 2019


Phosphite oxidoreductase (ptxD)

One restricting factor for cultivating microalgae is the high risk of contamination with bacteria, fungi or zooplankton. As our Team anticipates in building a bioreactor it is essential to sterilize the media and all containers which raise the costs of production. Loera-Quezada et al. (2016) found a way to bypass this problem by integrating Pseudomonas stutzeri WM88 gene ptxD in C. reinhardtii. It encodes a phosphite oxidoreductase which is able to oxidize phosphite into phosphate using NAD+ as a cofactor (White & Metcalf, 2007). The integration of ptxD into the genome of C. reinhardtii enables the use of phosphite as a sole phosphorus source which wasn’t possible before (Loera-Quezada et al., 2015). Loera-Quezada et al. (2016) presented a new way of microalgae production without the need for sterile conditions or antibiotics to avoid contamination with biological pollutants as they cannot use phosphite as a phosphorus source. In the future, this system could also be applied to open pond systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 946
  • 1000
    COMPATIBLE WITH RFC[1000]


MoClo Overhangs

This part with B3-B4 MoClo overhangs can be found here.

This part with B3-B3 MoClo overhangs can be found here.


References

  1. Loera‐Quezada, M. M., Leyva‐González, M. A., Velázquez‐Juárez, G., Sanchez‐Calderón, L., Do Nascimento, M., López‐Arredondo, D., & Herrera‐Estrella, L. (2016). A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae. Plant biotechnology journal, 14(10), 2066-2076.
  2. White, A. K., & Metcalf, W. W. (2007). Microbial metabolism of reduced phosphorus compounds. Annu. Rev. Microbiol., 61, 379-400.
  3. Loera-Quezada, M. M., Leyva-González, M. A., López-Arredondo, D., & Herrera-Estrella, L. (2015). Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae. Plant Science, 231, 124-130.