Difference between revisions of "Part:BBa K2957008"
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<partinfo>BBa_K2957099 short</partinfo>
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<partinfo>BBa_K2957008 short</partinfo>
  
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Contents: CMV, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance
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Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance
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What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression
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What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression  
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What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.
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How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts>.  
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What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement  
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in the immune system and is known to create neutrophil swarming. It has a NeonGreen tag attached.  
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How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2957099 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2957008 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2957099 parameters</partinfo>
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<partinfo>BBa_K2957008 parameters</partinfo>
 
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Revision as of 23:19, 19 October 2019


CMV, IL-8 with NeonGreen Tag (C-terminal)

Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1802
    Illegal SpeI site found at 92
    Illegal PstI site found at 1245
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
    Illegal PstI site found at 1245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1019
    Illegal BamHI site found at 1261
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1802
    Illegal SpeI site found at 92
    Illegal PstI site found at 1245
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1802
    Illegal SpeI site found at 92
    Illegal PstI site found at 1245
    Illegal AgeI site found at 1808
  • 1000
    COMPATIBLE WITH RFC[1000]