Difference between revisions of "Part:BBa K2984068"
 
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<partinfo>BBa_K2984068 short</partinfo>
 
<partinfo>BBa_K2984068 short</partinfo>
  
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Another strong constitutive promoter is the AR promoter that is composed of HSP70A and RBCS2 building a tandem promoter. Therefore the HSP70A (A) promoter is fused upstream of another promoter resulting in a stronger transgene expression (Schroda et al. 2000). The combination of the HSP70A (A) promoter and the RBCS2 (R) promoter building PAR has the positive effect that transcriptional gene silencing (TGS) is strongly reduced making this promoter popular for transgene expression (Schroda et al. 2002). This part was designed for C. reinhardtii
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Another strong constitutive promoter is the AR promoter that is composed of HSP70A and RBCS2 building a tandem promoter. Therefore the HSP70A (A) promoter is fused upstream of another promoter resulting in a stronger transgene expression (Schroda et al. 2000). The combination of the HSP70A (A) promoter and the RBCS2 (R) promoter building PAR has the positive effect that transcriptional gene silencing (TGS) is strongly reduced making this promoter popular for transgene expression (Schroda et al. 2002). This part was designed for <i>C. reinhardtii</i>.
  
 
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===MoClo Overhangs===
 
===MoClo Overhangs===
 
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This part with A1-A3 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">here</a>.
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<p>This part with A1-A3 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">here</a>.</p>
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<p>This part with A1-B1 MoClo overhangs can be found <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984025">here</a>.
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Latest revision as of 23:14, 19 October 2019


AR Promoter

Another strong constitutive promoter is the AR promoter that is composed of HSP70A and RBCS2 building a tandem promoter. Therefore the HSP70A (A) promoter is fused upstream of another promoter resulting in a stronger transgene expression (Schroda et al. 2000). The combination of the HSP70A (A) promoter and the RBCS2 (R) promoter building PAR has the positive effect that transcriptional gene silencing (TGS) is strongly reduced making this promoter popular for transgene expression (Schroda et al. 2002). This part was designed for C. reinhardtii.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 264
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


MoClo Overhangs

This part with A1-A3 MoClo overhangs can be found here.

This part with A1-B1 MoClo overhangs can be found here.



References

  1. Schroda, M., Blöcker, D., & Beck, C. F. (2000). The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas. The plant journal, 21(2), 121-131.