Difference between revisions of "Part:BBa K2984054"

 
 
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<partinfo>BBa_K2984054 short</partinfo>
 
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Level 1 construct containing secretion signal of arylsulfatase fused to the flourescent protein mVenus and glycomoduel. To influence the expression of mVenus, a bleomycin resistance gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the Psadintron. The construct ends with the Rbcs2 terminator.
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Level 1 construct containing secretion signal of arylsulfatase <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> fused to the flourescent protein <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984017">mVenus</a> and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984015">SP20</a> glycomodule. To influence the expression of mVenus, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984045">bleomycin resistance</a> gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984046">PsaDintron</a> for enhanced expression. The construct ends with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2984054 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984054 SequenceAndFeatures</partinfo>
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==Characterization==
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<figure>
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<img src="https://2019.igem.org/wiki/images/d/df/T--Humboldt_Berlin--Konstruk_19.jpeg" alt="colonies_total" width="500">
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<figcaption>Fig.1 - Image of a successful transformation in <i>E. coli</i> after ligation of the construct. The construct can then be isolated from <i>E. coli</i> to be transformed in <i>C. reinhardtii</i>.</figcaption>
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Latest revision as of 23:06, 19 October 2019


L1c-PsadIntron-bleRscp-ARS-YFP-SP20-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. Level 1 construct containing secretion signal of arylsulfatase ARS fused to the flourescent protein mVenus and the SP20 glycomodule. To influence the expression of mVenus, a bleomycin resistance gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the PsaDintron for enhanced expression. The construct ends with the Rbcs2 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2051
    Illegal NgoMIV site found at 1607
    Illegal NgoMIV site found at 2528
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

colonies_total
Fig.1 - Image of a successful transformation in E. coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii.