Difference between revisions of "Part:BBa K3105676:Design"
 
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===Design Notes===
 
===Design Notes===
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Functionality in Pichia pastoris <br>
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Functionality in <I>Pichia pastoris</I> <br>
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A double stop codon is introduced in the 5'end to stop protein translation. Residues of the pPICZ&#945;B vector are present before the actual terminator starts.
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−
 
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−
 
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===Source===
 
===Source===
  
 
Organism : <I>Pichia pastoris</I> <br>
 
Organism : <I>Pichia pastoris</I> <br>
−
DNA originates from the pPICZ&#945;B vector found in the invitrogen easy select <I>Pichia</I> expression kit
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DNA originates from the pPICZ&#945;B vector found in the Invitrogen easy select <I>Pichia</I> expression kit
  
 
===References===
 
===References===

Latest revision as of 23:04, 19 October 2019


pPICZαB fragment + AOX1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 131
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Functionality in Pichia pastoris

Source

Organism : Pichia pastoris
DNA originates from the pPICZαB vector found in the Invitrogen easy select Pichia expression kit

References