Difference between revisions of "Part:BBa K2984047"

Revision as of 22:58, 19 October 2019

L1c-AR-PETase-YFP-RbcS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the AR promoter, a B2-B2 linker, the PETase enzyme, a YFP mVenus marker and the Rbcs2 terminator. The part can be used to express PETase enzyme with a YFP marker in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression of the PETase.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1758
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 268
Illegal NheI site found at 1087
Illegal PstI site found at 1758
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 811
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1758
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1758
Illegal NgoMIV site found at 2235
1000
COMPATIBLE WITH RFC[1000]

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-This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">AR promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express PETase enzyme with a YFP marker in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression of the PETase.
+This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">AR promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express PETase enzyme with a YFP marker in <i>C. reinhardtii</i>. The YFP-mVenus tag can be used to detect the expression of the PETase.
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