Difference between revisions of "Part:BBa K2984039"

 
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984015">HA tag</a>, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.  
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984015">HA tag</a>, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express the PETase enzyme in <i>C. reinhardtii</i>. The 3xHA tag can be used to purify and detect the PETase enzyme.  
 
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Latest revision as of 22:34, 19 October 2019


L1c-PsaD-PETase-3xHA-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linker, the PETase enzyme, a HA tag, and the Rbcs2 terminator. The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1705
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1244
    Illegal PstI site found at 1705
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1705
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1705
  • 1000
    COMPATIBLE WITH RFC[1000]