Difference between revisions of "Part:BBa K2984039"
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<partinfo>BBa_K2984039 short</partinfo> | <partinfo>BBa_K2984039 short</partinfo> | ||
| − | This part is composed of the PsaD promoter | + | <html> |
| + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984015">HA tag</a>, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express the PETase enzyme in <i>C. reinhardtii</i>. The 3xHA tag can be used to purify and detect the PETase enzyme. | ||
| + | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 22:34, 19 October 2019
L1c-PsaD-PETase-3xHA-RBCS2
This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linker, the PETase enzyme, a HA tag, and the Rbcs2 terminator. The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1705
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1244
Illegal PstI site found at 1705 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal XhoI site found at 968 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1705
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1705
- 1000COMPATIBLE WITH RFC[1000]