Difference between revisions of "Part:BBa K2984037"
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| − | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Level 1 backbone carrying the gene for the Phosphite oxidoreductase (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984013">PtxD</a>) which allows | + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Level 1 backbone carrying the gene for the Phosphite oxidoreductase (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984013">PtxD</a>) which allows <i>C.reinhardtii</i> to grow on phosphite. The trancriptional unit starts with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and Ends with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The construct contains a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2</a> linker which connects the PsaD promoter to PtxD. |
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<img src="https://2019.igem.org/wiki/images/d/d2/T--Humboldt_Berlin--Konstruk_10.jpg" alt="colonies_total" width="500"> | <img src="https://2019.igem.org/wiki/images/d/d2/T--Humboldt_Berlin--Konstruk_10.jpg" alt="colonies_total" width="500"> | ||
| − | <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> | + | <figcaption>Fig.1 - Image of a successful transformation in <i>E. coli</i> after ligation of the construct. The construct can then be isolated from <i>E. coli</i> to be transformed in <i>C. reinhardtii</i></figcaption> |
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Latest revision as of 22:33, 19 October 2019
L1c-Psad-PtxD-Rbcs2
This vector is a part of the Chlamy-HUB-Collection. Level 1 backbone carrying the gene for the Phosphite oxidoreductase (PtxD) which allows C.reinhardtii to grow on phosphite. The trancriptional unit starts with the PsaD promoter and Ends with the Rbcs2 terminator. The construct contains a B2-B2 linker which connects the PsaD promoter to PtxD.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1790
- 1000COMPATIBLE WITH RFC[1000]
Characterization
