Difference between revisions of "Part:BBa K2984037"

 
 
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<partinfo>BBa_K2984037 short</partinfo>
 
<partinfo>BBa_K2984037 short</partinfo>
  
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Level 1 backbone carrying the gene for the Phosphite oxidoreductase (ptxD) which is supposed to allow  C.reinnhardtii to grow on phosphite. The trancriptional unit starts with the Psad Promoter and Ends with Rbcs2 Terminator.  
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Level 1 backbone carrying the gene for the Phosphite oxidoreductase (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984013">PtxD</a>) which allows <i>C.reinhardtii</i> to grow on phosphite. The trancriptional unit starts with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and Ends with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The construct contains a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2</a> linker which connects the PsaD promoter to PtxD.
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<partinfo>BBa_K2984037 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984037 SequenceAndFeatures</partinfo>
  
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==Characterization==
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<figure>
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<img src="https://2019.igem.org/wiki/images/d/d2/T--Humboldt_Berlin--Konstruk_10.jpg" alt="colonies_total" width="500">
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<figcaption>Fig.1 - Image of a successful transformation in <i>E. coli</i> after ligation of the construct. The construct can then be isolated from <i>E. coli</i> to be transformed in <i>C. reinhardtii</i></figcaption>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 22:33, 19 October 2019


L1c-Psad-PtxD-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. Level 1 backbone carrying the gene for the Phosphite oxidoreductase (PtxD) which allows C.reinhardtii to grow on phosphite. The trancriptional unit starts with the PsaD promoter and Ends with the Rbcs2 terminator. The construct contains a B2-B2 linker which connects the PsaD promoter to PtxD.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1790
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

colonies_total
Fig.1 - Image of a successful transformation in E. coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii