Difference between revisions of "Part:BBa K2984029"
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| − | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984001">GLE</a> secretion signal, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984016">SP20</a> glycomodule for enhanced secretion and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase. | + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984001">GLE</a> secretion signal, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984016">SP20</a> glycomodule for enhanced secretion and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The part can be used to express and secrete the PETase enzyme in <i>C. reinhardtii</i>. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase. |
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Latest revision as of 22:25, 19 October 2019
L1c-PsaD-GLE-PETase-YFP-SP20-RBCS2
This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, the GLE secretion signal, the PETase enzyme, a YFP mVenus marker, the SP20 glycomodule for enhanced secretion and the Rbcs2 terminator. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1775
Illegal PstI site found at 1989 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1314
Illegal PstI site found at 1775
Illegal PstI site found at 1989 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal XhoI site found at 1038 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1775
Illegal PstI site found at 1989 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1775
Illegal PstI site found at 1989
Illegal NgoMIV site found at 2466 - 1000COMPATIBLE WITH RFC[1000]
Characterization
